Membership is open only to organisations.

Member organisations must be working explicitly on human trafficking and/or related areas of migration, migrant labour, women's rights or sex workers' rights in at least one of the following areas: policy and advocacy, research, direct assistance, prevention.

Member organisations must be non-governmental.

Member organisations must adhere to GAATW's Basic Principles 

Member organisations must send the annual report of their work to the International Secretariat, to be made available to the whole Alliance.

Members who are working in the same country or region may choose to produce a joint report of the anti-trafficking work in their country/region.

Members, especially those who are working on other issues and are part of other human rights movements, are expected to make an effort to integrate trafficking issues into other movements and share this with other members.